Information about the Marijuana, Cannabis Testing Kits
These kits are “Thin Layer Chromatography” testing kits designed specifically for testing Marijuana sometimes called HPTLC. It is a patented and registered product, and was certified by the University of Leiden.
This kit will provide you with visual color indicators for the THC, THCV, CBG, CBNV, CBD substances and more in Medical Marijuana, There is testing for estimated THC % with visual indicators, and testing for acids and poisons
This kit is a good step in providing a balanced product that provides a way for growers and product producers to analyze their products during production to produce these products and an alternative to laboratory testing.
Here is some information that will come with the kit:
You get step by step directions with pictures of each step being taken
You get all the little reaction chambers and liquids, developing jar, capillary tubes, and more, needed to perform the entire testing.
Now included with every kit, Capillary tube extraction bulb, It makes it easier to take samples from reaction chambers.
You get color charts from previous certified base line tests to match with your results and a THC % chart to match estimated % of THC and other chemicals. Represented as a chart of dots – each dot represents a % point. The larger the dot the more %, you compare the dots from your results to the ones on the chart; it is a basic chromatography way of testing substances
These are some examples of the base line charts included,
I have added 2 see through Chart, (1-25 and 26-50) You can put your sample behind the chart and see the size of your sample though the dot
YOU WILL NEED: A good scale, latex gloves, a well ventilated working area and a scanner or digital camera to copy results. (Note: Air and Light degrade the results it is a good idea to scan the plate after finishing testing) You can save the image in any format including using photo shop, etc.
If you really want to get into a detailed way of defining your results (other than a visual comparison of your results to the charts ) and you know a little bit about photo editing programs, you can download for free: http://imagej.nih.gov/ij/ ( this is a photo editing program that can produce a numeric number evaluation, based on the area or Mean of substance dot) or try: http://www.justquantify.com/ . In both programs, you scan your results and scan the THC reference chart compare the density of the dots (shows in a numeric form) on the reference chart and compare them to the density numbers or area of your sample results that will give you a % value based on your chart.
You must read the entire manual before starting any testing. * Check out the video on the Main Page.
Above are 3 plates, the plate on the left is a “Cold Test using pure cannabis, we used 5 lanes. The one in the center is a Hot Test using pure cannabis, 5 lanes, (the CBD and CBG are brighter than can be seen here). The one on the right is a mix of different products, using a Hot Test. (The cookie test had very little cannabis in it that is why it shows up so faint on the plate)
When testing you can do “cold testing” which shows all the acids and the other relevant substances in the product up through the THC, CBD, CBG and CBN or you can do “hot testing” which moves more of the THC up to the top by dissolving some of the acids that would be left when a cold test is done (It is all explained in the directions.) The bottom line is, more THC % is tested by using the “hot testing method”. You can also divide the plate up so you can do hot and cold testing on one plate.
Labs do different types of testing- Gas chromatography (GC), High-performance liquid chromatography,(HPLC) and Thin Layer Chromatography ( TLC) which is what our kit does -With GC or HPLC the same plant can be tested 3 times and get 3 different results, also if you took the same sample to three different labs you would get three different results. Basically, there is no standard procedure from lab to lab for testing Medical Marijuana because of the lack of standard testing rules by the government.
3 Most common types of Lab testing
Thin layer chromatography (TLC) is a widely-used chromatography technique used to separate chemical compounds. It involves a stationary phase consisting of a thin layer of adsorbent material, usually silica gel, aluminum oxide, or cellulose immobilized onto a flat, inert carrier sheet. A liquid phase consisting of the solution to be separated dissolved in an appropriate solvent is drawn through the plate via capillary action, separating the experimental solution, whereas TLC can be configured for quantitative work
High-performance liquid chromatography is a form of column chromatography used frequently in analytical chemistry. HPLC is used to separate components of a mixture by using a variety of chemical interactions between the compounds within the substance being analyzed and the chromatography column.
A Gas Chromatograph is a chemical analysis instrument used for separating volatile and semi-volatile compounds in a complex sample. The stationary phase in GC is contained in a narrow -bored column and the mobile phase is a gas. The volatile and semi-volatile components of a sample mixture are separated based on their varying partition behavior between the mobile phase and the stationary phase.
The Reality is GC testing is not as good as we would like for testing of CBD, CBN, CBG, and some of the other substances in marijuana because the machines use heat to test these substances and they all evaporate at a lower temperature then THC. So you will get some results but they will not be that accurate and the coated tubes used in testing cost several hundred dollars a piece and are used for hundreds or thousands of tests before replacement, leading to problems from contamination and degradation of the column.
HPLC testing is complex and does do cold testing but if you are looking for the highest THC% then you must test HOT & COLD which most machines are not prepared to do. As with GC, the columns must be re-used many times, leading to contamination and degradation problems, also calibrating the machines should be done after every batch is tested and that is not done, all these systems need expensive equipment and are costly.
Our Method of Testing
TLC is a simple, quick, and inexpensive procedure that gives a quick answer as to how many components are in a mixture. TLC is also used to support the identity of a compound in a mixture when the Rf of a compound is compared with the Rf from the literature, In our case the cannabinoids can be identified by their specific color and specific location (Rf value) and order of appearance on the TLC plate, if carried out with the solvent system from the Kit.
A TLC plate is a sheet of glass which is coated with a thin layer of a solid adsorbent, silica. A small amount of the mixture to be analyzed is spotted near the bottom of this plate. The TLC plate is then placed in a shallow pool of a solvent in a developing chamber so that only the very bottom of the plate is in the liquid. This liquid, or eluent or carrier fluid, is the mobile phase, and it slowly rises up the TLC plate by capillary action.
As the solvent moves, it will pass the little spot of cannabinoid-oil micro-droplet of 2 ul or 4 ul that was applied to the bottom of the plate. An equilibrium is established for each component of the mixture between the molecules of that component which are absorbed on the solid and the molecules which are in solution. In principle, the components will differ in solubility and in the strength of their adsorption to the adsorbent and the other components will be carried farther up to the plate than the others. When the solvent has reached the top of the plate, the plate is removed from the developing chamber, dried (ventilation!). After drying, the separated components (Cannabinoids) now separated in the silica plates, will be visualized by spraying the plate with a dye. The dye is highly specific for Cannabinoids. Every principal Cannabinoid will display his own color! THC: wine red, CBD: bright yellow, CBG: orange, CBN: violet etc…
NOTE: If you are looking for Mold in your substances you need to just use a microscope.
Why should you use HPTLC for botanical identification?
Other techniques can address identification only in part. They either are limited to plant parts (microscopy), have difficulties with natural variability of botanical materials (IR techniques), or focus on the quantitative comparison of separated markers (HPLC).
All other techniques lack the versatility and flexibility of HPTLC for the analysis of botanicals. HPTLC is the ONLY single technique that offers a generally applicable approach to compliance with the FDA requirement of 100% botanical identification.
HPTLC is the primary chemical test for identification of all pharmacopoeia monographs on botanicals (including USP)
The acceptance and performance of HPTLC is demonstrated by the China HPTLC Atlas, AHP monographs, and the USP Dietary Supplement Compendium
HPTLC fingerprints do not just give a correlation coefficient but visually show the similarities and differences between samples and references on the same plate.
In addition to identity an HPTLC fingerprint can also give quantitative information (intensity of bands) about a sample for batch to batch comparison HPTLC can also be used for assays (potency), purity (adulteration), and stability studies (shelf life) HPLC testing is more time consuming and have limited detectability of non-UV absorbing compounds Unlike HPLC, HPTLC preserves the complete sample for analysis, so nothing is “hidden”.
Here is the way we see it. These kits give THC results that have been compared to a properly run and managed GC machine on a monthly basis and even clients with no testing backgrounds have tested with the kits and have had no more than 1% difference from the GC machine. Working with experienced chemists the conclusion has been that if you are going to make any type of products for sale, you will need to do many test on your substances for batching and dosage for medical use, or you are just going to retail products from liquids to hand creams, all these substances will have to be tested in the USA before going to retail.
It is not cost effective for any large or small company to spend a lot of money going to a lab for continues testing. Our kits used properly will give you consistent results if you use the correct amount stated for testing, so that you can create your substances or track your breeding for very little cost. Then once you have gotten your product to where you want it then you can go to a certified lab and have it tested so it can go to retail which will be the law that all substances will have to be tested by a certified lab at some point. Many labs are using our test kits already, some labs use our testing procedure and use no machines so instead of paying tons of money for initial testing using our kits is a sensible part of processing any type of cannabis product or substance, and some labs are telling clients to use the kits first and then come to them for final testing. It is a win win for everybody. Once you understand TLC testing you will see that it will become a standardized part of the cannabis industry.